Module of Plant tissue culture lectures and Practical experiments | Week | |
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1. |
Introduction of Plant tissue culture and basic knowledge about setting up Plant tissue culture laboratory |
1st |
1.1 |
Working of various equipments and their safe handling |
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2. |
Basic theoretical knowledge of micro and macro nutrients required for plant tissue culture medium. |
2nd |
2.1 |
Preparation of stocks solutions, hormones required for nutrient media |
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3. |
Theory about different plant tissue culture nutrient medium and its components. |
3rd |
3.1 |
Preparation of basal/specific nutrient MS medium for commercially important plant species with details of procedure and its sterilization by autoclaving |
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4. |
Theory about methodologies and optimized protocols in JUIT lab for tissue culture |
4th |
4.1 |
Surface sterilization and establishment of tissue cultures by using shoot apex |
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5. |
Theory about maintenance and analysis of growth parameters of shoot culture |
5th |
5.1 |
Optimization of incubation conditions and observation of growth of plantlets |
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Mid Term Evaluation (20 Maximum Marks) |
6th |
6. |
Theory about callus cultures with significance |
7th |
6.1 |
Media preparation for callus induction and regeneration |
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7. |
Micro propagation of different plant species by using different explants for large scale shoot proliferation |
8th |
7.1 |
Incubation of cultures under optimized culture room conditions and observation of growth parameters |
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8. |
Theory about in vitro and ex vitro root induction |
9th |
8.1 |
Media preparation for in vitro root induction |
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9. |
Theory about different articles and setups for hardening of tissue cultured plants in green house |
10th |
9.1 |
Transfer of plants for hardening and ex vitro rooting under green house conditions |
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10. |
Theory about hydroponic systems |
11th |
10.2 |
Media preparation and establishment of plants under the hydroponic system |
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11. |
Theory about production of phyto chemicals |
12th |
11.1 |
Establishment of cell/tissue culture systems for production of phytochemicals |
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Final Evaluation |
13th |
Reference:
1. Plant tissue culture: theory and Practice: S.S.Bhojwani and M K Razadan
2. Plant Cell and Tissue Culture ( A Lab Manual) : Jacob Reinert,Michael Magson Yeoman
3. Plant Tissue Culture Manual (Reference manual)